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Novel methodology to replicate clot analogs with diverse composition in acute ischemic stroke
  1. Sharon Duffy1,2,
  2. Michael Farrell3,
  3. Kevin McArdle2,
  4. John Thornton3,
  5. David Vale2,
  6. Eleanor Rainsford1,
  7. Liam Morris1,
  8. David S Liebeskind4,
  9. Eugene MacCarthy1,
  10. Michael Gilvarry2
  1. 1Mechanical and Industrial Engineering Department, Galway Medical Technologies Centre, Galway-Mayo Institute of Technology, Galway, Ireland
  2. 2Neuravi Ltd, Galway, Ireland
  3. 3Neuropathology Department, Beaumont Hospital, Dublin, Ireland
  4. 4Neurovascular Imaging Research Core and UCLA Stroke Centre, Los Angeles, California, USA
  1. Correspondence to S Duffy, Mechanical and Industrial Engineering Department, Galway Medical Technologies Centre, Galway-Mayo Institute of Technology, Old Dublin Rd, Galway, Ireland; sharon.duffy{at}research.gmit.ie

Abstract

Background Translational research on clot composition may be advanced by the use of clot analogs for the preclinical evaluation of mechanical thrombectomy devices. This work describes a novel set of clot analogs to represent a diverse range of fibrin and red blood cell (RBC) compositions for use in acute ischemic stroke (AIS) occlusion models.

Method Fresh whole blood obtained from ovine species was used to create seven different clot analog types. Five replicates were formed for each clot type. Varying amounts of whole blood constituents were mixed with thrombotic factors to create clots of varying compositions. Following histological processing, five sections from each clot were stained with H&E and Martius Scarlet Blue. Fibrin, RBC and white blood cell compositions were quantified.

Results Histological examination demonstrated that the clot types had a distinct RBC and fibrin composition. No significant difference in composition was shown between replicates (p>0.05), indicating that the method of clot formation was reproducible. Percentage fibrin composition of the clot types was 1%, 8%, 31%, 38%, 64%, 79%, and 100%. A significant difference in fibrin and RBC composition between clot types was observed (p<0.05).

Conclusions Seven different clot types were developed to replicate common AIS thrombi. These clot analogs may be beneficial for the preclinical evaluation of endovascular therapies, and may be applied to interventional technique training.

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