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Original research
Simultaneous R2* and quantitative susceptibility mapping measurement enables differentiation of thrombus hematocrit and age: an in vitro study at 3 T
  1. Spencer D Christiansen1,2,
  2. Junmin Liu2,
  3. Michael B Boffa3,
  4. Maria Drangova1,2
  1. 1 Department of Medical Biophysics, Schulich School of Medicine & Dentistry, The University of Western Ontario, London, Ontario, Canada
  2. 2 Imaging Research Laboratories, Robarts Research Institute, Schulich School of Medicine & Dentistry, The University of Western Ontario, London, Ontario, Canada
  3. 3 Department of Biochemistry, Schulich School of Medicine & Dentistry, The University of Western Ontario, London, Ontario, Canada
  1. Correspondence to Dr. Maria Drangova, Imaging Research Laboratories, Robarts Research Institute, Western University, 1151 Richmond Street, London, Ontario N6A 2B7, Canada; mdrangova{at}robarts.ca

Abstract

Background The efficacy of acute ischemic stroke treatment is affected by thrombus composition and age, yet no diagnostic method capable of quantitative thrombus characterization currently exists. This in vitro study evaluates the use of R2*, quantitative susceptibility mapping (QSM), and proton density fat fraction (FF) maps derived from a single gradient echo (GRE) MRI acquisition for characterizing clot of various hematocrit, as well as added calcified and lipidic components, throughout aging.

Methods Two thrombus phantoms containing porcine clots (10–60% hematocrit, one with added calcium or lard) were scanned serially throughout 6 days of aging. Three-dimensional multi-echo GRE imaging was used to generate R2*, QSM, and FF maps, from which mean values for all clots at every time point were obtained. Receiver operating characteristic analysis was used to derive thresholds differentiating acute from chronic clot, and measured R2* and QSM were tested for their ability to estimate clot hematocrit.

Results R2* and QSM varied minimally over the first 6 hours of aging (acute), and QSM was found to linearly relate to clot hematocrit. Beyond 6 hours (chronic), R2* and QSM increased considerably over time and hematocrit could be estimated from the R2*/QSM ratio. R2* and QSM thresholds of 22 s-1 and 0.165 ppm differentiated acute from chronic clots with a sensitivity/specificity of 100%/100% and 85%/92%, respectively. QSM and FF maps definitively distinguished calcium and lipid, respectively, from clots of any hematocrit and age.

Conclusions R2*, QSM, and FF from a single multi-echo GRE scan discriminated hematocrit and age, and distinguished calcification and lipid withinin vitro clot.

  • MRI
  • stroke
  • thrombectomy
  • thrombolysis
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Footnotes

  • Contributors SDC: study conception, data acquisition, data analysis, data interpretation, manuscript drafting, and manuscript editing. JL: study conception, data analysis, data interpretation, and manuscript editing. MBB: study conception and manuscript editing. MD: (guarantor) study conception, data interpretation, and manuscript editing.

  • Funding This work was supported in part by the Canadian Institutes of Health Research grant No CIHR #PJT 153411 and the Ontario Research Fund.

  • Competing interests None declared.

  • Provenance and peer review Not commissioned; externally peer reviewed.

  • Patient consent for publication Not required.

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