Article Text

Download PDFPDF
Original research
Arterial wall injury and miRNA expression induced by stent retriever thrombectomy under stenotic conditions in a dog model
  1. Jiangshan Deng1,2,
  2. Yiran Zhang1,
  3. Guangchen He1,
  4. Haitao Lu1,
  5. Yuwu Zhao2,
  6. Yuehua Li1,
  7. Yueqi Zhu1
  1. 1Radiology, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai, China
  2. 2Neurology, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai, China
  1. Correspondence to Dr Yueqi Zhu, Radiology, Shanghai Jiaotong University Affiliated Sixth People Hospital South Campus, Shanghai 200233, China; zhuyueqi{at}hotmail.com; Dr Yuehua Li, Radiology, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai 200233, China; liyuehua312{at}163.com

Abstract

Background Acute ischemic stroke can be caused by in situ stenotic vessel occlusion. In the present study, we compared the extent of arterial wall damage and miRNA expression following stent retriever use under normal and stenotic conditions.

Methods The stent retriever procedure was simulated in three dogs by the creation of four stenoses on each side of the common carotid artery (CCA) to allow five stent passages. Device safety was also assessed in normal control models by five passages through both CCAs. Device manipulation-related damage to the arterial walls was evaluated and compared between groups by angiography and pathological analysis. Real-time PCR was used to evaluate the differences in the expression of miRNAs between the two groups.

Results Twenty-four stenoses were created in three model dogs, and the mean stenosis rate was 65.58%±18.95%. Angiography revealed greater vasospasm in the stenotic group than in the non-stenotic group (1.17±0.17 vs 0.5±0.23; P=0.04). Pathological examination revealed that SR passage through the stenotic lumen caused higher injury scores (1.63±0.19 vs 0.25±0.09 for the non-stenotic lumen; P<0.001), more endothelial denudation (1.79±0.13 vs 0.58±0.13 for the non-stenotic lumen; P<0.001), and increased thrombus deposition (0.71±0.14 vs 0±0 for the non-stenotic lumen; P<0.001). miR21-3p, miR29-3p, and miR26a were upregulated in stenotic vessels compared with non-stenotic vessels after SR thrombectomy (P<0.001).

Conclusion In our model dogs, SR thrombectomy resulted in more severe tissue damage to the arterial wall under stenotic conditions than under non-stenotic conditions. The damage may have resulted from upregulation of miR21-3p, miR29-3p, and miR26a expression.

  • angiography
  • stent
  • thrombectomy

Statistics from Altmetric.com

Footnotes

  • JD and YZ contributed equally.

  • Contributors YZ and JD: acquisition of data, analysis and interpretation of data, and drafting of the manuscript. YZ: study concept and design, critical revision of the manuscript for important intellectual content, and study supervision. YL, HL, YZ and LW: acquisition of data and statistical analysis.

  • Funding This work was supported by grants: the National Natural Science Foundation of China (grant no.81671655), Shanghai Municipal Education Commission-Gaofeng Clinical Medicine Grant Support (No.20152528).

  • Competing interests None declared.

  • Patient consent for publication Not required.

  • Provenance and peer review Not commissioned; externally peer reviewed.

  • Data availability statement Data are available upon reasonable request.

Request Permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.