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Endothelialized silicone aneurysm models for in vitro evaluation of flow diverters
  1. Alyssa McCulloch1,
  2. Ashley Turcott1,
  3. Gabriella Graham1,
  4. Sergey Frenklakh2,
  5. Kristen O'Halloran Cardinal1
  1. 1Biomedical Engineering Department, Cal Poly, San Luis Obispo, California, USA
  2. 2Research and Development, Stryker Neurovascular Intervention, Fremont, California, USA
  1. Correspondence to Professor Kristen O'Halloran Cardinal, Biomedical Engineering Department, Cal Poly, San Luis Obispo, CA 93407, USA; kohallor{at}calpoly.edu

Abstract

Objective The goal of this work was to endothelialize silicone aneurysm tubes for use as in vitro models for evaluating endothelial cell interactions with neurovascular devices. The first objective was to establish consistent and confluent endothelial cell linings and to evaluate the silicone vessels over time. The second objective was to use these silicone vessels for flow diverter implantation and assessment.

Methods Silicone aneurysm tubes were coated with fibronectin and placed into individual bioreactor systems. Human umbilical vein endothelial cells were deposited within tubes to create silicone vessels, then cultivated on a peristaltic pump and harvested at 2, 5, 7, or 10 days to evaluate the endothelial cell lining. A subset of silicone aneurysm vessels was used for flow diverter implantation, and evaluated for cell coverage over device struts at 3 or 7 days after deployment.

Results Silicone vessels maintained confluent, PECAM-1 (platelet endothelial cell adhesion molecule 1) positive endothelial cell linings over time. These vessels facilitated and withstood flow diverter implantation, with robust cell linings disclosed after device deployment. Additionally, the endothelial cells responded to implanted devices through coverage of the flow diverter struts with increased cell coverage over the aneurysm seen at 7 days after deployment as compared with 3 days.

Conclusions Silicone aneurysm models can be endothelialized and successfully maintained in vitro over time. Furthermore, these silicone vessels can be used for flow diverter implantation and assessment.

  • aneurysm
  • device
  • flow diverter
http://creativecommons.org/licenses/by-nc/4.0/

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/.

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Footnotes

  • Contributors Concept planning and development of studies performed by AMC, GG, KOHC. Conduct of the study, aquisition and analysis of data performed by AMC, AT, SF. Writing of the article performed by AMC, KOHC. Critical review of the article performed by GG, AT, SF.

  • Funding The authors have not declared a specific grant for this research from any funding agency in the public, commercial or not-for-profit sectors.

  • Competing interests None declared.

  • Patient consent for publication Not required.

  • Provenance and peer review Not commissioned; externally peer reviewed.

  • Data availability statement Data are available upon reasonable request. Any detail related to the model or the results are available from the corresponding author.

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