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Original research
Fucoidan-coated coils improve healing in a rabbit elastase aneurysm model
  1. Zoltán Szatmáry1,2,
  2. Sylvia M Bardet2,
  3. Jérémy Mounier2,
  4. Kevin Janot2,3,
  5. Jonathan Cortese2,4,
  6. Marie Laure Perrin2,
  7. Cladue Couquet2,
  8. Guy Deniau5,
  9. Fanny Haquier5,6,
  10. Rodolphe Migneret7,8,
  11. Erwann Guenin9,
  12. Murielle Maire7,8,
  13. Jean-Baptist Michel7,
  14. Géraud Forestier1,2,
  15. Aurélien Le Flahec10,
  16. Claire Leger-Bretou10,
  17. Charbel Mounayer1,2,
  18. Frederic Chaubet7,8,
  19. Aymeric Rouchaud1,2
  1. 1Department of Interventional Neuroradiology, Limoges University, Limoges, France
  2. 2CNRS, XLIM, UMR 7252, Limoges University, Limoges, France
  3. 3Department of Interventional Neuroradiology, CHRU Tours CPU, Tours, France
  4. 4Department of Interventional Neuroradiology-NEURI Brain Vascular Center APHP, Hospital Bicetre, Le Kremlin-Bicêtre, France
  5. 5UMR CEA, CNRS 3685, NIMBE, LICSEN, Paris-Saclay University, Gif-sur-Yvette, France
  6. 6Department of Chemistry and Health and Life Sciences, CNAM, Paris, France
  7. 7INSERM, UMRS 1148, LVTS, Paris, France
  8. 8Institut Galilée, Sorbonne North Paris University, Villetaneuse, France
  9. 9Laboratoire TIMR, Centre de Recherche Royallieu Rue du Dr Schweitzer - CS 6031960200, Compiègne, France
  10. 10Limoges University, Limoges, France
  1. Correspondence to Dr Zoltán Szatmáry, Department of Interventional Neuroradiology, Limoges University, 87000 Limoges, Nouvelle Aquitaine, France; saintmaryz{at}hotmail.com

Abstract

Background Recanalization of coiled aneurysms remains unresolved. To limit aneurysm recanalization after embolization with coils, we propose an innovative approach to optimize aneurysm healing using fucoidan-coated coils.

Objective To evaluate the short-term efficacy and long-term safety of the new coil system with conventional angiography, histology, and multiphoton microscopy for follow-up of fibrosis and neointima formation.

Methods We conducted a feasibility study on rabbit elastase-induced aneurysms. Embolization was carried out with bare platinum coils, fucoidan-coated coils, or dextran-coated coils. Aneurysms were controlled after 1 month by digital subtraction angiography (DSA). Aneurysm samples were collected and processed for histological analysis. Aneurysm healing and fibrosis were measured by quantifying collagen according to the histological healing score by combining standard light microscopy and multiphoton imaging. We divided 27 rabbits into three groups: bare platinum group, fucoidan group, and dextran group as controls.

Results Angiographic grading showed a trend toward less recanalization in the fucoidan group, although there were no significant differences among the three groups (P=0.21). Histological healing was significantly different according to the presence of more collagen in the neck area of aneurysms in the fucoidan group versus the bare platinum group (P=0.011), but not in the dextran group. Histological index was significantly better at the aneurysm neck in the fucoidan group than in the bare platinum group (P=0.004). Collagen organization index was also significantly better in the fucoidan group than in the bare platinum group (P=0.007).

Conclusion This proof-of-concept study demonstrated the feasibility and efficacy of treatment with fucoidan-coated coils to improve aneurysm healing. The results in this rabbit in vivo model showed that fucoidan-coated coils have the potential to improve healing following endovascular treatment.

  • Aneurysm
  • Coil
  • Bioactive

Data availability statement

Data are available upon reasonable request. All data relevant to the study are included in the article or uploaded as supplementary information.

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Data availability statement

Data are available upon reasonable request. All data relevant to the study are included in the article or uploaded as supplementary information.

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Footnotes

  • Correction notice Since this paper first published, Erwann Guénin's, Murielle Maire's and Rodolphe Migneret's affiliations have been updated.

  • Contributors ZS, AR, FC, J-BM, CM conceived the presented idea. SMB verified the analytical methods and performed histology with JM. SMB, CM, FC, AR supervised the findings of this work. ZS, SMB, JM, KJ, AR, MLP, CC, GD, FH, RM, EG, MM carried out the experiments. ZS wrote the manuscript with support from SMB, J-BM, CM, FC, AR. All authors discussed the results and contributed to the final manuscript. Guarantor: ZS.

  • Competing interests None declared.

  • Provenance and peer review Not commissioned; externally peer reviewed.

  • Supplemental material This content has been supplied by the author(s). It has not been vetted by BMJ Publishing Group Limited (BMJ) and may not have been peer-reviewed. Any opinions or recommendations discussed are solely those of the author(s) and are not endorsed by BMJ. BMJ disclaims all liability and responsibility arising from any reliance placed on the content. Where the content includes any translated material, BMJ does not warrant the accuracy and reliability of the translations (including but not limited to local regulations, clinical guidelines, terminology, drug names and drug dosages), and is not responsible for any error and/or omissions arising from translation and adaptation or otherwise.