RT Journal Article
SR Electronic
T1 Novel methodology to replicate clot analogs with diverse composition in acute ischemic stroke
JF Journal of NeuroInterventional Surgery
JO J NeuroIntervent Surg
FD BMJ Publishing Group Ltd.
SP 486
OP 491
DO 10.1136/neurintsurg-2016-012308
VO 9
IS 5
A1 Duffy, Sharon
A1 Farrell, Michael
A1 McArdle, Kevin
A1 Thornton, John
A1 Vale, David
A1 Rainsford, Eleanor
A1 Morris, Liam
A1 Liebeskind, David S
A1 MacCarthy, Eugene
A1 Gilvarry, Michael
YR 2017
UL http://jnis.bmj.com/content/9/5/486.abstract
AB Background Translational research on clot composition may be advanced by the use of clot analogs for the preclinical evaluation of mechanical thrombectomy devices. This work describes a novel set of clot analogs to represent a diverse range of fibrin and red blood cell (RBC) compositions for use in acute ischemic stroke (AIS) occlusion models.Method Fresh whole blood obtained from ovine species was used to create seven different clot analog types. Five replicates were formed for each clot type. Varying amounts of whole blood constituents were mixed with thrombotic factors to create clots of varying compositions. Following histological processing, five sections from each clot were stained with H&E and Martius Scarlet Blue. Fibrin, RBC and white blood cell compositions were quantified.Results Histological examination demonstrated that the clot types had a distinct RBC and fibrin composition. No significant difference in composition was shown between replicates (p>0.05), indicating that the method of clot formation was reproducible. Percentage fibrin composition of the clot types was 1%, 8%, 31%, 38%, 64%, 79%, and 100%. A significant difference in fibrin and RBC composition between clot types was observed (p<0.05).Conclusions Seven different clot types were developed to replicate common AIS thrombi. These clot analogs may be beneficial for the preclinical evaluation of endovascular therapies, and may be applied to interventional technique training.