TY - JOUR T1 - A canine model of mechanical thrombectomy in stroke JF - Journal of NeuroInterventional Surgery JO - J NeuroIntervent Surg SP - 1243 LP - 1248 DO - 10.1136/neurintsurg-2019-014969 VL - 11 IS - 12 AU - Olivia W Brooks AU - Robert M King AU - Erez Nossek AU - Miklos Marosfoi AU - Jildaz Caroff AU - Ju-Yu Chueh AU - Ajit S Puri AU - Matthew J Gounis Y1 - 2019/12/01 UR - http://jnis.bmj.com/content/11/12/1243.abstract N2 - Purpose To develop a preclinical model of stroke with a large vessel occlusion treated with mechanical thrombectomy.Materials and methods An ischemic stroke model was created in dogs by the introduction of an autologous clot into the middle cerebral artery (MCA). A microcatheter was navigated to the clot and a stent retriever thrombectomy was performed with the goal to achieve Thrombolysis in Cerebral Ischemia (TICI) 2b/3 reperfusion. Perfusion and diffusion MRI was acquired after clot placement and following thrombectomy to monitor the progression of restricted diffusion as well as changes in ischemia as a result of mechanical thrombectomy. Post-mortem histology was done to confirm MCA territory infarct volume.Results Initial MCA occlusion with TICI 0 flow was documented in all six hound-cross dogs entered into the study. TICI 2b/3 revascularization was achieved with one thrombectomy pass in four of six animals (67%). Intra-procedural events including clot autolysis leading to spontaneous revascularization (n=1) and unresolved vasospasm (n=1) accounted for thrombectomy failure. In one case, iatrogenic trauma during microcatheter navigation resulted in a direct arteriovenous fistula at the level of the cavernous carotid. Analysis of MRI indicated that a volume of tissue from the initial perfusion deficit was spared with reperfusion following thrombectomy, and there was also a volume of tissue that infarcted between MRI and ultimate recanalization.Conclusion We describe a large animal stroke model in which mechanical thrombectomy can be performed. This model may facilitate, in a preclinical setting, optimization of complex multimodal stroke treatment paradigms for clinical translation. ER -