TY - JOUR T1 - Arterial wall injury and miRNA expression induced by stent retriever thrombectomy under stenotic conditions in a dog model JF - Journal of NeuroInterventional Surgery JO - J NeuroIntervent Surg SP - 563 LP - 567 DO - 10.1136/neurintsurg-2020-016347 VL - 13 IS - 6 AU - Jiangshan Deng AU - Yiran Zhang AU - Guangchen He AU - Haitao Lu AU - Yuwu Zhao AU - Yuehua Li AU - Yueqi Zhu Y1 - 2021/06/01 UR - http://jnis.bmj.com/content/13/6/563.abstract N2 - Background Acute ischemic stroke can be caused by in situ stenotic vessel occlusion. In the present study, we compared the extent of arterial wall damage and miRNA expression following stent retriever use under normal and stenotic conditions.Methods The stent retriever procedure was simulated in three dogs by the creation of four stenoses on each side of the common carotid artery (CCA) to allow five stent passages. Device safety was also assessed in normal control models by five passages through both CCAs. Device manipulation-related damage to the arterial walls was evaluated and compared between groups by angiography and pathological analysis. Real-time PCR was used to evaluate the differences in the expression of miRNAs between the two groups.Results Twenty-four stenoses were created in three model dogs, and the mean stenosis rate was 65.58%±18.95%. Angiography revealed greater vasospasm in the stenotic group than in the non-stenotic group (1.17±0.17 vs 0.5±0.23; P=0.04). Pathological examination revealed that SR passage through the stenotic lumen caused higher injury scores (1.63±0.19 vs 0.25±0.09 for the non-stenotic lumen; P<0.001), more endothelial denudation (1.79±0.13 vs 0.58±0.13 for the non-stenotic lumen; P<0.001), and increased thrombus deposition (0.71±0.14 vs 0±0 for the non-stenotic lumen; P<0.001). miR21-3p, miR29-3p, and miR26a were upregulated in stenotic vessels compared with non-stenotic vessels after SR thrombectomy (P<0.001).Conclusion In our model dogs, SR thrombectomy resulted in more severe tissue damage to the arterial wall under stenotic conditions than under non-stenotic conditions. The damage may have resulted from upregulation of miR21-3p, miR29-3p, and miR26a expression.Data are available upon reasonable request. ER -