TY - JOUR T1 - Histological evaluation of acute ischemic stroke thrombi may indicate the occurrence of vessel wall injury during mechanical thrombectomy JF - Journal of NeuroInterventional Surgery JO - J NeuroIntervent Surg DO - 10.1136/neurintsurg-2021-017310 SP - neurintsurg-2021-017310 AU - Oana Madalina Mereuta AU - Mehdi Abbasi AU - Seán Fitzgerald AU - Daying Dai AU - Ram Kadirvel AU - Ricardo A Hanel AU - Albert J Yoo AU - Mohammed A Almekhlafi AU - Kennith F Layton AU - Josser E Delgado Almandoz AU - Peter Kvamme AU - Vitor Mendes Pereira AU - Babak S Jahromi AU - Raul G Nogueira AU - Matthew J Gounis AU - Biraj Patel AU - Amin Aghaebrahim AU - Eric Sauvageau AU - Parita Bhuva AU - Jazba Soomro AU - Andrew M Demchuk AU - Ike C Thacker AU - Yasha Kayan AU - Alexander Copelan AU - Pouya Nazari AU - Donald Robert Cantrell AU - Diogo C Haussen AU - Alhamza R Al-Bayati AU - Mahmoud Mohammaden AU - Leonardo Pisani AU - Gabriel Martins Rodrigues AU - Ajit S Puri AU - John Entwistle AU - Alexander Meves AU - Jorge L Arturo Larco AU - Luis Savastano AU - Harry J Cloft AU - David F Kallmes AU - Karen M Doyle AU - Waleed Brinjikji Y1 - 2021/05/10 UR - http://jnis.bmj.com/content/early/2021/08/24/neurintsurg-2021-017310.abstract N2 - Background Several animal studies have demonstrated that mechanical thrombectomy (MT) for acute ischemic stroke (AIS) may cause vessel wall injury (VWI). However, the histological changes in human cerebral arteries following MT are difficult to determine.Objective To investigate the occurrence of VWI during MT by histological and immunohistochemical evaluation of AIS clots.Methods As part of the multicenter STRIP registry, 277 clots from 237 patients were analyzed using Martius Scarlett Blue stain and immunohistochemistry for CD34 (endothelial cells) and smooth muscle actin (smooth muscle cells).Results MT devices used were aspiration catheters (100 cases), stentriever (101 cases), and both (36 cases). VWI was found in 33/277 clots (12%). There was no significant correlation between VWI and MT device. The degree of damage varied from grade I (mild intimal damage, 24 clots), to grade II (relevant intimal and subintimal damage, 3 clots), and III (severe injury, 6 clots). VWI clots contained significantly more erythrocytes (p=0.006*) and less platelets/other (p=0.005*) than non-VWI clots suggesting soft thrombus material.Thrombolysis correlated with a lower rate of VWI (p=0.04*). VWI cases showed a significantly higher number of passes (2 [1–4] vs 1 [1–3], p=0.028*) and poorer recanalization outcome (p=0.01*) than cases without VWI.Conclusions Histological markers of VWI were present in 12% of AIS thrombi, suggesting that VWI might be related to MT. VWI was associated with soft thrombus consistency, higher number of passes and poorer revascularization outcome. There was no significant correlation between VWI and MT device.Data are available from the corresponding author upon reasonable request. ER -