PT  - JOURNAL ARTICLE
AU  - Jeong-Min Kim
AU  - Jun-Soo Byun
AU  - Jiah Kim
AU  - Moo-Seok Park
AU  - Soon Auck Hong
AU  - Taek-Kyun Nam
AU  - Hyun Ho Choi
AU  - Sungguan Hong
AU  - Su-Hyun Han
AU  - Hae-Bong Jeong
AU  - Kwang-Yeol Park
AU  - Hye Ryoun Kim
TI  - Analysis of microRNA signatures in ischemic stroke thrombus
AID  - 10.1136/neurintsurg-2021-017597
DP  - 2022 May 01
TA  - Journal of NeuroInterventional Surgery
PG  - 469--474
VI  - 14
IP  - 5
4099  - http://jnis.bmj.com/content/14/5/469.short
4100  - http://jnis.bmj.com/content/14/5/469.full
SO  - J NeuroIntervent Surg2022 May 01; 14
AB  - Background We investigated the microRNA expression pattern from thrombus retrieved by mechanical thrombectomy in acute stroke patients to understand the stroke mechanism.Methods This study included acute ischemic stroke patients who had undergone intra-arterial thrombectomy at Chung-Ang University Hospital in Seoul, Korea between February 2016 and March 2019. The thrombus was retrieved and stored at −70℃ after obtaining informed consent. MicroRNA microarray analysis was performed for the patients with identified stroke mechanisms including (1) large artery atherosclerosis, (2) cardioembolism with atrial fibrillation, and (3) cardioembolism with valvular heart disease. The microRNAs derived from microarray analysis were validated by quantitative real-time polymerase chain reaction (qRT-PCR) from different patient populations. The correlation analysis was performed between microRNA levels and laboratory data to understand the functional relevance of the altered microRNA.Results In total, 55 thrombi were obtained from 74 patients, and the microRNAs were analyzed in 45 samples. Microarray analysis of 2578 microRNAs revealed that 50 microRNAs were significantly altered among the three groups. Validation using qRT-PCR showed that miR-378f and miR-450b-5p were significantly elevated among the cardioembolic thrombi; both microRNAs were inversely correlated with the ejection fraction from echocardiography. Thrombi from patients with early neurological deterioration exhibited higher levels of miR-93-5p and lower levels of miR-629-5p than those from neurologically stable patients.Conclusions The microRNA expression pattern can provide information regarding the mechanism of stroke by reflecting the underlying pathological status of the organ from which the thrombus was derived.The data that support the findings of this study are available from the corresponding author, upon reasonable request.