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A failure of matrix metalloproteinase inhibition in the prevention of rat intracranial aneurysm formation

  • Interventional Neuroradiology
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Abstract

We tested the hypothesis that nonspecific matrix metalloproteinase (MMP) inhibition with doxycycline would decrease the incidence of intracranial aneurysm formation in a rat aneurysm model. We performed common carotid artery ligation on 96 Long-Evans rats. A treatment group of 48 animals was chosen at random to receive oral doxycycline (3 mg/kg) in addition to standard rat chow, and the control group of 48 animals received standard rat chow only. The major circle of Willis arteries was dissected at 1 year following carotid ligation, and the proportions of animals with aneurysms were compared between groups using Fisher’s exact test. Four animals given oral doxycycline and ten control animals expired before 1 year. Of the examined animals, eight saccular intracranial aneurysms were found in 8 of 45 animals which had received doxycycline (17.8%) and seven saccular intracranial aneurysms were found in 7 of 37 control animals (18.9%). There was no significant difference in aneurysm formation between the doxycycline-treated and control groups (P=0.894). Nonspecific MMP inhibition with doxycycline is not effective in preventing intracranial aneurysm formation in a rat model.

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Acknowledgements

A Radiological Society of North America Research Resident Grant supported this work. This work was performed in the Department of Radiology, University of Virginia, Charlottesville, VA, USA. We sincerely thank Kevin S. Lee, Ph.D., University of Virginia, for invaluable advice and assistance in perfusion-fixation.

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Authors and Affiliations

  1. Department of Radiology, Mayo Clinic and Foundation, Mayo Building, E2, 200 1st St. SW, Rochester, MN, 55905, USA

    T. J. Kaufmann & D. F. Kallmes

  2. Asheville Radiology Associates, Asheville, NC, USA

    W. F. Marx

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  1. T. J. Kaufmann
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  2. W. F. Marx
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  3. D. F. Kallmes
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Correspondence to T. J. Kaufmann.

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The authors have no personal or institutional financial interest in drugs, materials or devices described in this submission

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Kaufmann, T.J., Marx, W.F. & Kallmes, D.F. A failure of matrix metalloproteinase inhibition in the prevention of rat intracranial aneurysm formation. Neuroradiology 48, 190–195 (2006). https://doi.org/10.1007/s00234-005-0025-4

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  • DOI: https://doi.org/10.1007/s00234-005-0025-4

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