Cell attachment to collagen: Isolation of a cell attachment mutant

doi:10.1016/0014-4827(77)90074-X

Experimental Cell Research

Volume 104, Issue 1, January 1977, Pages 119-125

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Abstract

A cell attachment mutant of the Chinese hamster ovary cell line (CHO) was isolated with the aid of an enrichment procedure. The mutant isolated, CHO^att−, was found to be defective in its attachment to collagen in two respects. (1) The mutant requires 100- and 1 000-fold more Mg²⁺ and Ca²⁺, respectively, than wild-type cells for 50% cell attachment to collagen; (2) the mutant displays only 5% of the affinity of wild-type cells for a serum cell attachment protein. CHO^att−, which was selected for non-attachment to collagen, is also altered in its ability to attach to synthetic substrata, such as glass and plastic. Collagen adhesive revertants of CHO^att− were isolated. The revertants differed morphologically from wild type CHO cells. The adhesive response to Ca²⁺, Mg²⁺, and cell attachment protein in each revertant was either partially or fully restored.

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^☆: This work was supported, in part, by grants CA 190 17 and GM 214 33 from the NIH.

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Cell attachment to collagen: Isolation of a cell attachment mutant☆

Abstract

Exp cell res

Exp cell res

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